With years of experience, Creative Biolabs is a leading company specializing in offering binding range determination assay services. Our position as an end-to-end company providing high-quality analytical services provides us with a great advantage in helping clients around the world. With professional knowledge and an advanced platform, our scientists are fully confident in completing the most challenging binding range determination assay to facilitate the progress of your project.
The cell walls of Gram-positive bacteria are rich in carbohydrate structures. In general, carbohydrates elicit a poor immune response. The lysibody approach provides new directions to address this shortcoming. As an engineered molecule, lysibodies combine a phage-derived high-affinity domain and the Fc effector portion of a human IgG antibody. Evidence suggests that lysibodies are closely linked to the treatment and prevention of infection with drug-resistant pathogens.
In general, it is necessary to characterize all lysibodies to confirm their therapeutic potential. The most important step is to examine the binding capacity to specific bacterial strains and the host range of the lysibodies. Characterizing the host range could also test whether the lysibody could be used to treat infections with other pathogenic strains, making it easier to find the most effective method.
At Creative Biolabs, assays relying on fluorescence microscopy to test lysibody binding to the cell walls of different bacteria are available. Fluorescent dyes are frequently used in detection. The novel dyes we employed are very lightfast and have high fluorescence quantum yields. Furthermore, red-emitting fluorescent markers are very valuable in biological microscopy because they minimize cellular autofluorescence and increase the flexibility of multicolor experiments. In the assay, firstly, the strain to be detected needs to be selected. Bacterial cells are then fixed, attached to microscope coverslips, and blocked. Cells are then incubated with a specific lysibody followed by an anti-human IgG Fc fluorescence conjugate. According to the results, the lysosomal host binding range is determined.
Fig.1 Binding range of AtlA lysibody. (Raz, 2017)
Our dedicated experts have sufficient knowledge of the binding range determination assay. We will choose the most suitable assay solution according to your project needs.
We have state-of-the-art equipment as well as professional experimental technology.
Our customer-oriented seasoned scientists provide high-quality binding range determination services with fast turnaround times.
We will provide valid reports with repeatable experimental results.
Creative Biolabs is committed to providing cost-effective binding range determination assay services to support novel lysibodies with reliable scientific validation. If you are interested in the field of binding range determination, our experts can provide you with the required analytical data. Please feel free to contact us for more information and a bespoke option for your project.
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