Lysibody-induced Complement Deposition Determination Assay

In recent years, the role of lysibody in inducing complement deposition has attracted widespread attention. As an expert in the field of lysibody, Creative Biolabs is committed to providing global customers with a full range of lysibody-induced complement deposition determination assay services, from experimental design to result analysis.

Overview of Lysibody

The cell walls of Gram-positive bacteria contain abundant carbohydrate structures, however, the poor immune response to carbohydrates limits their function. The advent of the lysibody provides an attractive tool to address this challenge. As an engineered molecule, lysibodies combine a phage-derived binding domain with the Fc portion of a human immunoglobulin G (IgG) antibody. Thus, lysibodies are closely linked to the efficient immunity of bacterial surface carbohydrates. Furthermore, the Fc moiety attracts complement and professional phagocytes and promotes bacterial clearance in vitro and mouse sepsis models.

Principles and Procedures

Deposition of complement on the surface of pathogens is an important mechanism for their clearance by phagocytes. In general, fluorescence microscopy is a crucial tool to determine whether lysibodies can induce complement fragment fixation on the surface of Gram-positive bacteria. Lysibodies play a crucial role in inducing complement deposition on the cell surface of Gram-positive bacteria. The cells of Gram-positive bacteria are attached to specially treated coverslips and incubated with lysibodies. The cells are then incubated with human complement, washed, fixed, and blocked. The experimental results are presented by microscopy using a C3-specific antibody and a fluorescent conjugate to assess the extent of complement deposition on the Gram-positive bacteria.

Fig.1 Lysibodies induce deposition of complement on the surface of S. aureus cells. (Raz, 2017)

Services at Creative Biolabs

Creative Biolabs is a leading global service provider dedicated to discovering effective and rapid methods to advance lysibody research. We have established an advanced platform with multiple state-of-the-art facilities. In addition, our experts have extensive experience and expertise in customizing high-affinity lysibodies according to your project needs. Creative Biolabs is committed to providing cost-effective one-stop lysibody-induced complement deposition determination assay services to global customers.

Why Choose Us?

With a full range of customer service programs on offer, we are confident to help design a bespoke service package to facilitate the progress of your project. Advantages of our services include:

• Constructing lysibodies specific to Gram-positive bacteria
• Various techniques are available, including enzyme-linked immunosorbent assay (ELISA) and fluorescence microscopy
• Precise and accurate detection methods are developed
• Multidisciplinary experts ensure the accuracy of the analysis results

Understanding the relationship between lysibody and complement deposition contributes to revealing the biological properties and mechanism of action of lysibodies. With extensive expertise in lysibody, Creative Biolabs offers lysibody-induced complement deposition determination assay services to assist customers in characterizing the interaction between lysibodies and complement and screening the best lysibody for therapeutic use. Please contact us for professional assistance and reasonable solutions.

Reference:

1. Raz, A.; et al. Lysibodies are IgG Fc fusions with lysin binding domains targeting Staphylococcus aureus wall carbohydrates for effective phagocytosis. Proceedings of the National Academy of Sciences. 2017, 114(18): 4781-4786.